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The foundation of any multicolor flow experiment is always proper compensation. This is typically the first step in an analysis - relying on quality single stained compensation controls, either from beads or cells. Learn to: gate positive and negative control populations, troubleshoot the compensation platform, add/remove parameters, and check the calculated spillover matrix. Compensation can be tricky for novice and advanced cytometrists alike, but with a little practice, FlowJo's compensation Wizard feature will make this process a breeze.

Sep 21, 2017 10:00 AM in Pacific Time (US and Canada)

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